Distribution and Contamination Rate Analysis of Blood Culture in Children_West China Medical Journal

Authors：

 JIANGWen-qiang ,  LIHong-xia , LUOJun , DENGQiang , LIFen

Clinical Laboratory of Mianyang Central Hospital, Mianyang, Sichuan 621000, P. R. China;

Corresponding?author：

LIHong-xia, Email: lihongxia1118@163.com

Keywords：

Automated blood culture system; Children; Blood culture; Bacteria; Contamination rate

DOI：

10.7507/1002-0179.20140214

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the distribution of bacteria detected from blood culture of pediatric patients and to observe the blood culture contamination rate. Methods A total of 6 530 blood samples, collected from January 2011 to December 2012 were detected by BacT/Alert 3D automated blood culture system. We found out the contamination bacteria according to clinical data, laboratory data and microbiology knowledge. Results A total of 314 bacteria strains were isolated from 6 530 blood samples, and the positive rate was 4.8%, 228 of which were gram-positive bacteria. The isolates were mainly coagulase-negative staphylococci (43.9%), followed by Staphylococcus aureus (2.9%). In addition, 86 cases were gram-negative bacteria, the majority of which were Escherichia coli (9.6%), followed by Klebsiella pneumonia (8.3%). The overall blood culture contamination rate was 49.7% (156 bacteria were identified). The top two were coagulase-negative staphylococci (31.2%), followed by Bacillus sp. (6.4%). Conclusion The contamination rate is high in children's blood culture, and coagulase-negative staphylococci are the main bacteria. It's necessary to use clinical data and laboratory data to determine its clinical significance, and avoid unnecessary use of antibiotics.

Citation： JIANGWen-qiang, LIHong-xia, LUOJun, DENGQiang, LIFen. Distribution and Contamination Rate Analysis of Blood Culture in Children. West China Medical Journal, 2014, 29(4): 718-720. doi: 10.7507/1002-0179.20140214 Copy

1.	Yanai M, Uehara Y, Yagoshi M, et al. Interpretation of the results of blood culture and interventional role by clinical laboratory physicians[J]. Rinsho Byori, 2006, 54(10):1059-1065.
2.	Lee CC, Lin WJ, Shih HI, et al. Clinical significance of potential contaminants in blood cultures among patients in a medical center[J]. J Microbiol Immunol Infect, 2007, 40(5):438-444.
3.	Kristóf K, Kocsis E, Nagy K. Clinical microbiology of early-onset and late-onset neonatal sepsis, particularly among preterm babies[J]. Acta Microbiol Immunol Hung, 2009, 56(1):21-51.
4.	喬寧, 喻華. BacT/Alert240全自動血培養儀臨床應用評價[J]. 實用醫院臨床雜志, 2009, 6(1):61-62.
5.	陶傳敏, 溫曉波, 鄺玉, 等. BacT/Alert 240全自動血培養儀的臨床應用及評價[J]. 華西醫學, 2002, 17(3):366-368.
6.	曹慧玲, 李岷, 魏源華, 等. 血培養病原菌分布及污染菌判定的實驗室檢查[J]. 國際檢驗醫學雜志, 2011, 32(18):2067-2068, 2071.
7.	張斗星, 張林, 李宗光, 等. 探討血培養陽性結果在新生兒血流感染中的意義[J]. 安徽醫學, 2012, 33(10):1290-1292.
8.	Richter SS, Beekmann SE, Croco JL, et al. Minimizing the workup of blood culture contaminants:implementation and evaluation of a laboratory-based algorithm[J]. J Clin Microbiol, 2002, 40(7):2437-2444.
9.	王進, 肖永紅. 2006-2007年Mohnarin血流感染病原菌構成及耐藥性[J]. 中華醫院感染學雜志, 2008, 18(9):1238-1242.
10.	徐修禮, 楊春龍, 樊新, 等. 血培養標本中病原菌分布及其耐藥性分析[J]. 中華醫院感染學雜志, 2008, 18(10):1456-1459.
11.	孫寶君, 鄒琳. 血培養陽性病原菌分布及變化趨勢的臨床分析[J]. 解放軍醫學雜志, 2005, 30(5):430-431.
12.	Favre B, Hugonnet S, Correa L, et al. Nosocomial bacteremia:clinical significance of a single blood culture positive for coagulase-negative staphylococci[J]. Infect Control Hosp Epidemiol, 2005, 26(8):697-702.
13.	李天嬌, 鐘海英, 郭紅荔, 等. 新生兒血培養出凝固酶陰性葡萄球菌的分析[J]. 中國實驗診斷學, 2010, 14(9):1451-1453.
14.	王輝, 張悅嫻, 謝秀麗, 等. 血培養凝固酶陰性葡萄球菌陽性的臨床意義[J]. 中國抗感染化療雜志, 2001, 1(2):79-82.
15.	陳杏春. 血培養對新生兒敗血癥的診斷價值[J]. 廣西醫學, 2010, 32(8):970-972.
16.	王堅鏹, 湯瑾, 莊亦暉, 等. 雙套血培養對提高血流感染檢出率和鑒別污染的評價[J]. 中國感染與化療雜志, 2012, 12(6):440-442.
17.	毛美麗, 汪瑞忠. 血培養單、雙側采血陽性率比較及病原菌分布和耐藥性分析[J]. 檢驗醫學, 2009, 24(12):911-913.
18.	顧海彤, 黃艷飛, 孫宇峰, 等. 不同種類微生物血培養陽性報警時間的臨床意義探討[J]. 中國實驗診斷學, 2011, 15(11):1882-1884.
19.	Roth A, Wiklund AE, P?lsson AS, et al. Reducing blood culture contamination by a simple informational intervention[J]. J Clin Microbiol, 2010, 48(12):4552-4558.

1. Yanai M, Uehara Y, Yagoshi M, et al. Interpretation of the results of blood culture and interventional role by clinical laboratory physicians[J]. Rinsho Byori, 2006, 54(10):1059-1065.
2. Lee CC, Lin WJ, Shih HI, et al. Clinical significance of potential contaminants in blood cultures among patients in a medical center[J]. J Microbiol Immunol Infect, 2007, 40(5):438-444.
3. Kristóf K, Kocsis E, Nagy K. Clinical microbiology of early-onset and late-onset neonatal sepsis, particularly among preterm babies[J]. Acta Microbiol Immunol Hung, 2009, 56(1):21-51.
4. 喬寧, 喻華. BacT/Alert240全自動血培養儀臨床應用評價[J]. 實用醫院臨床雜志, 2009, 6(1):61-62.
5. 陶傳敏, 溫曉波, 鄺玉, 等. BacT/Alert 240全自動血培養儀的臨床應用及評價[J]. 華西醫學, 2002, 17(3):366-368.
6. 曹慧玲, 李岷, 魏源華, 等. 血培養病原菌分布及污染菌判定的實驗室檢查[J]. 國際檢驗醫學雜志, 2011, 32(18):2067-2068, 2071.
7. 張斗星, 張林, 李宗光, 等. 探討血培養陽性結果在新生兒血流感染中的意義[J]. 安徽醫學, 2012, 33(10):1290-1292.
8. Richter SS, Beekmann SE, Croco JL, et al. Minimizing the workup of blood culture contaminants:implementation and evaluation of a laboratory-based algorithm[J]. J Clin Microbiol, 2002, 40(7):2437-2444.
9. 王進, 肖永紅. 2006-2007年Mohnarin血流感染病原菌構成及耐藥性[J]. 中華醫院感染學雜志, 2008, 18(9):1238-1242.
10. 徐修禮, 楊春龍, 樊新, 等. 血培養標本中病原菌分布及其耐藥性分析[J]. 中華醫院感染學雜志, 2008, 18(10):1456-1459.
11. 孫寶君, 鄒琳. 血培養陽性病原菌分布及變化趨勢的臨床分析[J]. 解放軍醫學雜志, 2005, 30(5):430-431.
12. Favre B, Hugonnet S, Correa L, et al. Nosocomial bacteremia:clinical significance of a single blood culture positive for coagulase-negative staphylococci[J]. Infect Control Hosp Epidemiol, 2005, 26(8):697-702.
13. 李天嬌, 鐘海英, 郭紅荔, 等. 新生兒血培養出凝固酶陰性葡萄球菌的分析[J]. 中國實驗診斷學, 2010, 14(9):1451-1453.
14. 王輝, 張悅嫻, 謝秀麗, 等. 血培養凝固酶陰性葡萄球菌陽性的臨床意義[J]. 中國抗感染化療雜志, 2001, 1(2):79-82.
15. 陳杏春. 血培養對新生兒敗血癥的診斷價值[J]. 廣西醫學, 2010, 32(8):970-972.
16. 王堅鏹, 湯瑾, 莊亦暉, 等. 雙套血培養對提高血流感染檢出率和鑒別污染的評價[J]. 中國感染與化療雜志, 2012, 12(6):440-442.
17. 毛美麗, 汪瑞忠. 血培養單、雙側采血陽性率比較及病原菌分布和耐藥性分析[J]. 檢驗醫學, 2009, 24(12):911-913.
18. 顧海彤, 黃艷飛, 孫宇峰, 等. 不同種類微生物血培養陽性報警時間的臨床意義探討[J]. 中國實驗診斷學, 2011, 15(11):1882-1884.
19. Roth A, Wiklund AE, P?lsson AS, et al. Reducing blood culture contamination by a simple informational intervention[J]. J Clin Microbiol, 2010, 48(12):4552-4558.

Previous Article
Analysis of Clinical Common Pathogenic Bacteria Distribution and Drug Resistance
Next Article
Application of Dexmedetomidine Combined with Etomidate for Elderly Patient Undergoing thyroidectomy

West China Medical Journal

Distribution and Contamination Rate Analysis of Blood Culture in Children

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content