Experimental Study of Dexamethasone Treating in Non-freezing Cold Injury of Sciatic Nerves_West China Medical Journal

Authors：

 LIHao ¹ , XUMin ²

1. Department of Neurology, the First People's Hospital of Yibin, Yibin, Sichuan 644000, P. R. China;
2. Department of Neurology, Xuanwu Hospital of the Capital Medical University, Beijing, 100053, P. R. China;

Corresponding?author：

Keywords：

Non-freezing cold injury; Sciatic nerve; Dexamethasone

DOI：

10.7507/1002-0179.20140068

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Objective To observe the changes of microstructure of rats'sciatic nerves with non-freezing cold injury after treated with dexamethasone. Methods Twelve male Wistar rats were randomly divided into cooling group and treating group.Unilateral sciatic nerves of the rats in the cooling group received cooling treatment with 3-5℃ for 2 hours;while unilateral sciatic nerves of the rats in the treating group received cooling treatment with 3-5℃ for 2 hours and underwent the celiac injection with dexamethasone in addition.The other sciatic nerves were exposed,as the control.The bilateral sciatic nerves of rats in each group were harvested after 24 hours.The microstructure of nerves was examined under the light microscope and electron microscope. Results Light microscopic examination revealed extensive myelinated fibre degeneration in form of giant empty axons or shrunken dark axons on the first day after cooled.And the endoneurial capillary lumen was narrowed because of swollen endothelial cells.After the treatment,myelinated fibre degeneration was still similar to that before the treatment,but the endoneurial capillary lumen and endothelial cells were normal.By electron microscopy,sciatic nerves showed extensive myelinated fiber degeneration,and swollen endothelial cells.But unmyelinated fibers and tight junction were preserved on the first days after cooled.Aggregated red cells and platelet thrombus were not found.After the treatment,myelinated fibre degeneration was still similar to that before the treatment.Unmyelinated fibers and tight junction were preserved.The endoneurial capillary lumen and endothelial cells were normal. Conclusion After the treatment,the damage of endoneurial capillary had improved,but myelinated fiber degeneration was similar to that before the treatment.It suggested that dexamethasone may only improve the vascular system in non-freezing cold injury of sciatic nerve.

Citation： LIHao, XUMin. Experimental Study of Dexamethasone Treating in Non-freezing Cold Injury of Sciatic Nerves. West China Medical Journal, 2014, 29(2): 219-222. doi: 10.7507/1002-0179.20140068 Copy

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1. 宋波,張涌泉.北方海島部隊新兵訓練凍傷情況分析[J].白求恩軍醫學院學報,2008,6(6):363-364.
2. 應焱燕,朱銀潮,徐榮,等.2008年寧波地區雨雪冰凍災害所致傷害的流行病學調查[J].衛生研究,2009,38(4):463-464.
3. 劉玉紅,楊偉娜,楊光元,等.高原高寒地區凍傷200例致殘相關因素調查分析[J].臨床軍醫雜志,2007,35(1):94-95.
4. Jia J,Pollock M.The pathogenesis of non-freezing cold nerve injury.Observations in the rat[J].Brain,1997,120(Pt 4):631-646.
5. Jia J,Pollock M.Cold nerve injury is enhanced by intermittent cooling[J].Muscle Nerve,1999,22(12):1644-1652.
6. Jia JP,Pollock M,Jia JM.Cold injury to nerves is not due to ischaemia alone[J].Brain,1998,121(Pt 5):989-1001.
7. 張鍇,李積勝.寒冷環境對機體的影響及其機制[J].國外醫學·衛生學分冊,2006,33(4):212-215.
8. Dyck PJ,Giannini C.Pathologic alterations in the diabetic neuropathies of humans:a review[J].J Neuropathol Exp Neurol,1996,55(12):1181-1193.
9. Omura K,Ohbayashi M,Sano M,et al.The recovery of blood-nerve barrier in crush nerve injury--a quantitative analysis utilizing immunohistochemistry[J].Brain Res,2004,1001(1/2):13-21.
10. 耿志偉,徐敏,吳憂,等.氧自由基與大鼠坐骨神經非凍結性冷損傷[J].腦與神經疾病雜志,2008,16(5):598-601.
11. 耿志偉,張錦,佟曉燕,等.對坐骨神經局部溫度變化過程中氧自由基的研究[J].黑龍江醫學,2009,33(7):493-496.
12. 劉嘉瀛.氧自由基在凍傷發病中的作用[J].解放軍預防醫學雜志,1998,16(6):72-74.
13. Myers RR,Powell HC,Heckman HM,et al.Biophysical and Pathological effects of cryogenic nerve lesion[J].Ann Neurol,1981,10(5):478-485.
14. Olsson Y.Mast cells in the nervous system[J].Int Rev Cytol,1968,24:27-70.
15. 繆建云,練克儉.糖皮質激素治療神經損傷的研究進展[J].中國骨與關節損傷雜志,2009,24(6):572-574.
16. 陳新謙,金有豫,湯光,等.新編藥物學[M].北京:人民衛生出版社,1992:426.
17. 李浩,徐敏,耿志偉,等.巨噬細胞與坐骨神經非凍結性冷損傷[J].腦與神經疾病雜志,2010,18(6):432-435.

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