The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry_West China Medical Journal

Authors：

HUANG Xiaoqin ¹ , Jiangyangzeren ² , LAI Li ² , GUAN Yan ²

1. National Medical School; 2. Preclinical School, Chengdu Traditional Chinese Medicine University, Chengdu, Sichuan 611137, P. R. China;

Corresponding?author：

Keywords：

巴桑母酥油; 放射治療; 化學療法; 骨髓細胞; 造血干細胞; 造血祖細胞

DOI：

10.7507/1002-0179.20130377

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的　觀察藏醫滋補方“巴桑母酥油丸”對放射線-化學復合損傷小鼠骨髓不同細胞群增殖能力的影響，探討其促進放射線-化學復合損傷機體外周血象恢復的機制。方法　采用造血祖細胞集落分析方法、流式細胞術，檢測灌胃巴桑母酥油丸后放射線-化學復合損傷小鼠骨髓細胞中早期紅系祖細胞（CFU-E）、晚期造血祖細胞（BFU-E）、粒-巨噬系祖細胞（CFU-GM）、巨核系祖細胞（CFU-Meg）集落產率、骨髓細胞增殖周期各時相細胞比例、造血干細胞抗原-1（Sca-1）免疫表型陽性細胞數變化情況。結果　巴桑母酥油丸組骨髓細胞S+G2/M期細胞比例高于生理鹽水組（P＜0.05）和自然恢復的空白組（P＜0.01）；CFU-E、BFU-E、CFU-GM集落產率高于生理鹽水組和空白組（P＜0.05）；CFU-Meg集落產率、Sca-1+細胞數在各組間差異無統計學意義（P＞0.05）。結論　巴桑母酥油丸對放射線-化學復合損傷小鼠骨髓細胞具有促進增殖的作用，這可能是其促進放射線-化學復合損傷機體外周血象恢復的途徑，但對不同階段、不同系別的造血細胞其促進作用不同。

Citation： HUANG Xiaoqin,Jiangyangzeren,LAI Li,GUAN Yan. The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry. West China Medical Journal, 2013, 28(8): 1207-1210. doi: 10.7507/1002-0179.20130377 Copy

1.	宇妥·云丹袞波. 醫學四續[M]. 李多美, 譯. 西安: 西安出版社, 2000: 631.
2.	曾科,牛挺. 電離輻射的危害及放射線檢查的健康風險[J].華西醫學, 2011, 26(12): 1814-1817.
3.	第司·桑吉嘉措. 藍琉璃[M]. 毛繼祖, 卡洛, 毛韶玲, 譯. 上海: 上海科學技術出版社, 2012: 617.
4.	黃曉芹, 賴先榮, 降央澤仁, 等. 藏藥巴桑母酥油丸對放、化復合損傷“血虛證”小鼠造血功能干預初探[J]. 中藥與臨床, 2010, 1(2): 38-39.
5.	黃曉芹, 降央澤仁, 祝彼得. 放、化復合損傷至血虛證小鼠腎臟EPO、脾臟EPO-R、骨髓GM-CSF mRNA表達改變[J]. 成都中醫藥大學學報, 2009, 32(3): 56-60.
6.	Kaimakis P, Crisan M, Dzierzak E. The biochemistry of hematopoietic stem cell development[J]. Biochim Biophys Acta, 2013, 1830(2): 2395-2403.
7.	Van Zant G, Liang Y. Concise review: hematopoietic stem cell aging, life span, and transplantation[J]. Stem Cells Transl Med, 2012, 1(9): 651-657.
8.	Walasek MA, van Os R, de Haan G. Hematopoietic stem cell expansion: challenges and opportunities[J]. Ann N Y Acad Sci, 2012, 1266: 138-150.
9.	Takizawa H, Schanz U, Manz MG. Ex vivo expansion of hematopoietic stem cells: mission accomplished?[J]. Swiss Med Wkly, 2011, 141: 13316.
10.	Holmes C, Stanford WL. Concise review: stem cell antigen-1: expression, function, and enigma[J]. Stem Cells, 2007, 25(6): 1339-1347.
11.	Zhang CC, Lodish HF. Murine hematopoietic stem cells change their surface phenotype during ex vivo expansion[J]. Blood, 2005, 105(11): 4314-1420.
12.	Spangrude GJ, Brooks DM. Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells[J]. Blood, 1993, 82(11): 3327-3332.
13.	Uchida N, Weissman IL. Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow[J]. J Exp Med, 1992, 175(1): 175-184.
14.	Ito CY, Li CY, Bernstein A, et al. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice[J]. Blood, 2003, 101(2): 517-523.
15.	Bradfute SB, Graubert TA, Goodell MA. Roles of Sca-1 in hematopoietic stem/progenitor cell function[J]. Exp Hematol, 2005, 33(7): 836-843.
16.	Balasubramani SP, Venkatasubramanian P, Kukkupuni SK, et al. Plant-based Rasayana drugs from Ayurveda[J]. Chin J Intear Med, 2011, 17(2): 88-94.
17.	Vayalil PK, Kuttan G, Kuttan R. Protective effects of Rasayanas on cyclophosphamideand radiation-induced damage[J]. J Altern Complement Med, 2002, 8(6): 787-796.
18.	張忠誠. 印度阿育吠陀醫學研究現狀[J]. 國外醫藥·植物藥分冊, 2000, 15(1): 18-22.
19.	李秀麗, 葉峰, 俞騰飛. 余甘子的藥理研究進展[J]. 時珍國醫國藥, 2006, 17(2): 266-267.
20.	王紅玲, 熊順軍, 洪艷, 等. 黃精多糖對全身60Coγ射線照射小鼠外周血細胞數量及功能的影響[J]. 數理醫藥學雜志, 2000, 13(6): 493-494.
21.	劉萍. 黃精多糖對老齡大鼠老化相關酶的影響[J]. 華西醫學, 2010, 25(7): 1259-1261.
22.	帝瑪爾·丹增彭措. 晶珠本草[M]. 毛繼祖, 林鵬程, 吉守祥, 等, 譯. 上海: 上海科學技術出版社, 2012: 217.

1. 　宇妥·云丹袞波. 醫學四續[M]. 李多美, 譯. 西安: 西安出版社, 2000: 631.
2. 　曾科,牛挺. 電離輻射的危害及放射線檢查的健康風險[J].華西醫學, 2011, 26(12): 1814-1817.
3. 　第司·桑吉嘉措. 藍琉璃[M]. 毛繼祖, 卡洛, 毛韶玲, 譯. 上海: 上海科學技術出版社, 2012: 617.
4. 　黃曉芹, 賴先榮, 降央澤仁, 等. 藏藥巴桑母酥油丸對放、化復合損傷“血虛證”小鼠造血功能干預初探[J]. 中藥與臨床, 2010, 1(2): 38-39.
5. 　黃曉芹, 降央澤仁, 祝彼得. 放、化復合損傷至血虛證小鼠腎臟EPO、脾臟EPO-R、骨髓GM-CSF mRNA表達改變[J]. 成都中醫藥大學學報, 2009, 32(3): 56-60.
6. 　Kaimakis P, Crisan M, Dzierzak E. The biochemistry of hematopoietic stem cell development[J]. Biochim Biophys Acta, 2013, 1830(2): 2395-2403.
7. 　Van Zant G, Liang Y. Concise review: hematopoietic stem cell aging, life span, and transplantation[J]. Stem Cells Transl Med, 2012, 1(9): 651-657.
8. 　Walasek MA, van Os R, de Haan G. Hematopoietic stem cell expansion: challenges and opportunities[J]. Ann N Y Acad Sci, 2012, 1266: 138-150.
9. 　Takizawa H, Schanz U, Manz MG. Ex vivo expansion of hematopoietic stem cells: mission accomplished?[J]. Swiss Med Wkly, 2011, 141: 13316.
10. 　Holmes C, Stanford WL. Concise review: stem cell antigen-1: expression, function, and enigma[J]. Stem Cells, 2007, 25(6): 1339-1347.
11. 　Zhang CC, Lodish HF. Murine hematopoietic stem cells change their surface phenotype during ex vivo expansion[J]. Blood, 2005, 105(11): 4314-1420.
12. 　Spangrude GJ, Brooks DM. Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells[J]. Blood, 1993, 82(11): 3327-3332.
13. 　Uchida N, Weissman IL. Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow[J]. J Exp Med, 1992, 175(1): 175-184.
14. 　Ito CY, Li CY, Bernstein A, et al. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice[J]. Blood, 2003, 101(2): 517-523.
15. 　Bradfute SB, Graubert TA, Goodell MA. Roles of Sca-1 in hematopoietic stem/progenitor cell function[J]. Exp Hematol, 2005, 33(7): 836-843.
16. 　Balasubramani SP, Venkatasubramanian P, Kukkupuni SK, et al. Plant-based Rasayana drugs from Ayurveda[J]. Chin J Intear Med, 2011, 17(2): 88-94.
17. 　Vayalil PK, Kuttan G, Kuttan R. Protective effects of Rasayanas on cyclophosphamideand radiation-induced damage[J]. J Altern Complement Med, 2002, 8(6): 787-796.
18. 　張忠誠. 印度阿育吠陀醫學研究現狀[J]. 國外醫藥·植物藥分冊, 2000, 15(1): 18-22.
19. 　李秀麗, 葉峰, 俞騰飛. 余甘子的藥理研究進展[J]. 時珍國醫國藥, 2006, 17(2): 266-267.
20. 　王紅玲, 熊順軍, 洪艷, 等. 黃精多糖對全身60Coγ射線照射小鼠外周血細胞數量及功能的影響[J]. 數理醫藥學雜志, 2000, 13(6): 493-494.
21. 　劉萍. 黃精多糖對老齡大鼠老化相關酶的影響[J]. 華西醫學, 2010, 25(7): 1259-1261.
22. 　帝瑪爾·丹增彭措. 晶珠本草[M]. 毛繼祖, 林鵬程, 吉守祥, 等, 譯. 上海: 上海科學技術出版社, 2012: 217.

Previous Article
Arthroscopically Assisted Suturing to Repair Bucket Handle Meniscal Tear
Next Article
Mechanism of Radioresistance Function of Water Etracts from Coix Lachrymal-jobi

West China Medical Journal

The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content