Construction of controllable polyethylene glycol bioactive coating with hemocompatibility from the surface of modified glass substrate_Journal of Biomedical Engineering

Authors：

 WEI Yu ¹ , ZHANG Jingxun ¹ , ZHANG Yuzhong ² , FENG Xiantao ¹ , YANG Xuanhuang ¹

1. School of Chemical and Pharmaceutical Engineering, Huanghuai University, Zhumadian, Henan 463000, P.R.China;
2. Henan Jinkai Investment Holding Group, Kaifeng, Henan 475002, P.R.China;

Corresponding?author：

WEI Yu, Email: wuxinren83@163.com

Keywords：

surface-initiated atom transfer radical polymerization; poly(ethylene glycol) methacrylate; glycidyl methacrylate; active polymer coating

DOI：

10.7507/1001-5515.201705032

Video：

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Abstract Full text Figures/Tables Video References Cited by

A diblock copolymer, poly(ethylene glycol) methacrylate-block-glycidyl methacrylate (PEGMA-GMA), was prepared on glass substrate by surface-initiated atom transfer radical polymerization (SI-ATRP), and endothelial specific peptide Arg-Glu-Asp-Val (REDV) was immobilized at the end of the PEGMA-GMA polymer brush by ring opening reaction through the rich epoxy groups in the GMA. The structure and hydrophilicity of the polymer brushes were characterized by static water contact angle, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The results showed that the REDV modified copolymer brushes were successfully constructed on the glass substrates. The REDV peptide immobilized onto surface was quantitatively characterized by ultraviolet–visible spectroscopy (UV-VIS). The blood compatibility of the coating was characterized by recalcification time and platelet adhesion assay. The results showed that the polymer coating had good blood compatibility. The multifunctional active polymer coating with PEGMA and peptide produced an excellent prospect in surface construction with endothelial cells selectivity.

Citation： WEI Yu, ZHANG Jingxun, ZHANG Yuzhong, FENG Xiantao, YANG Xuanhuang. Construction of controllable polyethylene glycol bioactive coating with hemocompatibility from the surface of modified glass substrate. Journal of Biomedical Engineering, 2019, 36(2): 260-266. doi: 10.7507/1001-5515.201705032 Copy

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2.	Wei Y, Zhang J X, Ji Y, et al. REDV Rapamycin-loaded polymer combinations as a coordinated strategy to enhance endothelial cells selectivity for a stent system. Colloids Surf B Biointerfaces, 2015, 136: 1166-1173.
3.	Pana C J, Wang J, Huang N. Preparation, characterization and in vitro anticoagulation of emodin-eluting controlled biodegradable stent coatings. Colloids Surf B Biointerfaces, 2010, 77: 155-160.
4.	Chen Jialong, Cao Jianjun, Wang Juan, et al. Biofunctionalization of titanium with PEG and anti-CD34 for hemocompatibility and stimulated endothelialization. J Colloid Interface Sci, 2012, 368(1): 636-647.
5.	魏雨, 張景迅, 范娟娟, 等. 心血管支架表面改性及應用. 生物醫學工程學雜志, 2016, 33(3): 593-597.
6.	Chang Hao, Liu Xiqiu, Hu Mi, et al. Substrate stiffness combined with hepatocyte growth factor modulates endothelial cell behavior. Biomacromolecules, 2016, 17(9): 2767-2776.
7.	Wei Y, Ji Y, Xiao L L. Surface engineering of cardiovascular stent with endothelial cellselectivity for in vivo re-endothelialisation. Biomaterials, 2013, 34(11): 2588-2599.
8.	魏雨, 張景迅, 范娟娟, 等. 基于聚乙二醇和不同多肽構建的生物涂層及其內皮細胞選擇性研究. 高分子學報, 2016(1): 118-124.
9.	Huang C F. Surface-initiated atom transfer radical polymerization for applications in sensors, non-biofouling surfaces and adsorbents. Polym J, 2016, 48(4, SI): 341-350.
10.	Xu F J, Neoh K G, Kang E T. Bioactive surfaces and biomaterials via atom transfer radical polymerization. Prog Polym Sci, 2009, 34(8): 719-761.
11.	Wang Jingjing, Wei Jun. Hydrogel brushes grafted from stainless steel via surface-initiated atom transfer radical polymerization for marine antifouling. Appl Surf Sci, 2016, 382: 202-216.
12.	Wei Yu, Zhang Jingxun, Li Haolie, et al. Multifunctional copolymer coating of polyethylene glycol, glycidyl methacrylate, and REDV to enhance the selectivity of endothelial cells. J Biomater Sci Polym Ed, 2015, 26(18): 1357-1371.
13.	Li Xin, Wang Mengmeng, Wang Lei, et al. Block copolymer modified surfaces for conjugation of biomacromolecules with control of quantity and activity. Langmuir, 2013, 29(4): 1122-1128.
14.	鄭軍, 李丹, 袁琳, 等. 表面接枝嵌段共聚物刷實現內皮細胞的選擇性黏附. 高分子學報, 2013(8): 1108-1114.
15.	Liu Yingshuai, Wang Wei, Hu Weihua, et al. Highly sensitive poly[glycidyl methacrylate-co-poly(ethylene glycol) methacrylate] brush-based flow-through microarray immunoassay device. Biomed Microdevices, 2011, 13(4): 769-777.
16.	Iwata R, Satoh R, Iwasaki Y, et al. Covalent immobilization of antibody fragments on well-defined polymer brushes via site-directed method. Colloids Surf B Biointerfaces, 2008, 62(2): 288-298.
17.	Pimpha N, Chaleawlert-umpon S, Chruewkamlow N, et al. Preparation of anti-CD4 monoclonal antibody-conjugated magnetic poly(glycidyl methacrylate) particles and their application on CD4(+) lymphocyte separation. Talanta, 2011, 84(1): 89-97.
18.	Xu F J, Zhu Y, Chai M Y, et al. Comparison of ethanolamine/ethylenediamine-functionalized poly(glycidyl methacrylate) for efficient gene delivery. Acta Biomater, 2011, 7(8): 3131-3140.
19.	Plouffe B D, Njoka D N, Harris J, et al. Peptide-mediated selective adhesion of smooth muscle and endothelial cells in microfluidic shear flow. Langmuir, 2007, 23: 5050-5055.
20.	Wei Yu, Ji Ying, Xiao Linlin, et al. Different complex surfaces of polyethyleneglycol (PEG) and REDV ligand to enhance the endothelial cells selectivity over smooth muscle cells. Colloids Surf B Biointerfaces, 2011, 84(2): 369-378.
21.	魏雨. 內皮細胞選擇性心血管支架仿生涂層材料的研究. 杭州: 浙江大學, 2011.
22.	Wu Zhaoqiang, Chen Hong, Liu Xiaoli, et al. Protein adsorption on poly(n-vinylpyrrolidone)-modified silicon surfaces prepared by surface-initiated atom transfer radical polymerization. Langmuir, 2009, 25(5): 2900-2906.
23.	Xu F J, Cai Q J, Li Y L, et al. Covalent immobilization of glucose oxidase on well-defined poly(glycidyl methacrylate)-Si(111) hybrids from surface-initiated atom-transfer radical polymerization. Biomacromolecules, 2005, 6(2): 1012-1020.

1. Weng Yajun, Chen Junying, Tu Qiufen, et al. Biomimetic modification of metallic cardiovascular biomaterials: from function mimicking to endothelialization in vivo. Interface Focus, 2012, 2(3): 356-365.
2. Wei Y, Zhang J X, Ji Y, et al. REDV Rapamycin-loaded polymer combinations as a coordinated strategy to enhance endothelial cells selectivity for a stent system. Colloids Surf B Biointerfaces, 2015, 136: 1166-1173.
3. Pana C J, Wang J, Huang N. Preparation, characterization and in vitro anticoagulation of emodin-eluting controlled biodegradable stent coatings. Colloids Surf B Biointerfaces, 2010, 77: 155-160.
4. Chen Jialong, Cao Jianjun, Wang Juan, et al. Biofunctionalization of titanium with PEG and anti-CD34 for hemocompatibility and stimulated endothelialization. J Colloid Interface Sci, 2012, 368(1): 636-647.
5. 魏雨, 張景迅, 范娟娟, 等. 心血管支架表面改性及應用. 生物醫學工程學雜志, 2016, 33(3): 593-597.
6. Chang Hao, Liu Xiqiu, Hu Mi, et al. Substrate stiffness combined with hepatocyte growth factor modulates endothelial cell behavior. Biomacromolecules, 2016, 17(9): 2767-2776.
7. Wei Y, Ji Y, Xiao L L. Surface engineering of cardiovascular stent with endothelial cellselectivity for in vivo re-endothelialisation. Biomaterials, 2013, 34(11): 2588-2599.
8. 魏雨, 張景迅, 范娟娟, 等. 基于聚乙二醇和不同多肽構建的生物涂層及其內皮細胞選擇性研究. 高分子學報, 2016(1): 118-124.
9. Huang C F. Surface-initiated atom transfer radical polymerization for applications in sensors, non-biofouling surfaces and adsorbents. Polym J, 2016, 48(4, SI): 341-350.
10. Xu F J, Neoh K G, Kang E T. Bioactive surfaces and biomaterials via atom transfer radical polymerization. Prog Polym Sci, 2009, 34(8): 719-761.
11. Wang Jingjing, Wei Jun. Hydrogel brushes grafted from stainless steel via surface-initiated atom transfer radical polymerization for marine antifouling. Appl Surf Sci, 2016, 382: 202-216.
12. Wei Yu, Zhang Jingxun, Li Haolie, et al. Multifunctional copolymer coating of polyethylene glycol, glycidyl methacrylate, and REDV to enhance the selectivity of endothelial cells. J Biomater Sci Polym Ed, 2015, 26(18): 1357-1371.
13. Li Xin, Wang Mengmeng, Wang Lei, et al. Block copolymer modified surfaces for conjugation of biomacromolecules with control of quantity and activity. Langmuir, 2013, 29(4): 1122-1128.
14. 鄭軍, 李丹, 袁琳, 等. 表面接枝嵌段共聚物刷實現內皮細胞的選擇性黏附. 高分子學報, 2013(8): 1108-1114.
15. Liu Yingshuai, Wang Wei, Hu Weihua, et al. Highly sensitive poly[glycidyl methacrylate-co-poly(ethylene glycol) methacrylate] brush-based flow-through microarray immunoassay device. Biomed Microdevices, 2011, 13(4): 769-777.
16. Iwata R, Satoh R, Iwasaki Y, et al. Covalent immobilization of antibody fragments on well-defined polymer brushes via site-directed method. Colloids Surf B Biointerfaces, 2008, 62(2): 288-298.
17. Pimpha N, Chaleawlert-umpon S, Chruewkamlow N, et al. Preparation of anti-CD4 monoclonal antibody-conjugated magnetic poly(glycidyl methacrylate) particles and their application on CD4(+) lymphocyte separation. Talanta, 2011, 84(1): 89-97.
18. Xu F J, Zhu Y, Chai M Y, et al. Comparison of ethanolamine/ethylenediamine-functionalized poly(glycidyl methacrylate) for efficient gene delivery. Acta Biomater, 2011, 7(8): 3131-3140.
19. Plouffe B D, Njoka D N, Harris J, et al. Peptide-mediated selective adhesion of smooth muscle and endothelial cells in microfluidic shear flow. Langmuir, 2007, 23: 5050-5055.
20. Wei Yu, Ji Ying, Xiao Linlin, et al. Different complex surfaces of polyethyleneglycol (PEG) and REDV ligand to enhance the endothelial cells selectivity over smooth muscle cells. Colloids Surf B Biointerfaces, 2011, 84(2): 369-378.
21. 魏雨. 內皮細胞選擇性心血管支架仿生涂層材料的研究. 杭州: 浙江大學, 2011.
22. Wu Zhaoqiang, Chen Hong, Liu Xiaoli, et al. Protein adsorption on poly(n-vinylpyrrolidone)-modified silicon surfaces prepared by surface-initiated atom transfer radical polymerization. Langmuir, 2009, 25(5): 2900-2906.
23. Xu F J, Cai Q J, Li Y L, et al. Covalent immobilization of glucose oxidase on well-defined poly(glycidyl methacrylate)-Si(111) hybrids from surface-initiated atom-transfer radical polymerization. Biomacromolecules, 2005, 6(2): 1012-1020.

Journal of Biomedical Engineering

Construction of controllable polyethylene glycol bioactive coating with hemocompatibility from the surface of modified glass substrate

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