Non-leaching, broad-spectrum antibacterial poly (ε-caprolactone)/gelatin-QAS nanofiberous membranes for wound dressing_Journal of Biomedical Engineering

Authors：

SHI Rui ¹ , GENG Huan ² , ZHANG Jingshuang ¹ ,  TIAN Wei ^1,3

1. Laboratory of Implant/Intervention Materials for Bone Functional Reconstruction, Beijing Institution of Traumatology and Orthopedics, Beijing 100035, P.R.China;
2. Beijing Laboratory of Biomaterials, Beijing University of Chemical Technology, Beijing 100029, P.R.China;
3. Beijing Jishuitan Hospital, the Fourth Medical Clinical Hospital of Peking University, Beijing 100035, P.R.China;

Corresponding?author：

TIAN Wei, Email: tianweijst@vip.163.com

Keywords：

electrospinning; antibacterial; wound dressing; quaternary ammonium salts

DOI：

10.7507/1001-5515.201701020

Video：

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5–20 wt% trimethoxysilylpropyl octadecyldimethyl ammonium chloride (QAS) was used to modify Poly (ε-caprolactone) (PCL)-gelatin hybrid to fabricate non-leaching antibacterial nanofiber membranes (PG-Q) by electrospinning. The results from scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated that the QAS leaded to phase separation between the QAS and PCL. Hydrophilic test demonstrated that the PG-Q nanofiber membranes had hydrophobic surface, which was help for peeling off the dressing from the wound. Additionally, the physical and chemical cross-linking between the QAS/PCL and QAS/gelatin were confirmed by Fourier transform infrared (FTIR), which were good for long lasting antibacterial effect. The PG-Q membranes also showed excellent cell-biocompatibility. Furthermore, compared with pure PCL nanofiber membrane, the PG-Q nanofiber membranes, especially PG-Q15 (QAS: 15 wt%) and PG-Q20 (QAS: 20 wt%), showed a considerable increase in the bacteriostatic rate of S. aureus and P. aeruginosa (more than 99% after 12 h). Therefore, electrospinning non-leaching antibacterial nanofiber membranes could be an optimal choice for antibacterial wound dressing.

Citation： SHI Rui, GENG Huan, ZHANG Jingshuang, TIAN Wei. Non-leaching, broad-spectrum antibacterial poly (ε-caprolactone)/gelatin-QAS nanofiberous membranes for wound dressing. Journal of Biomedical Engineering, 2018, 35(3): 396-402. doi: 10.7507/1001-5515.201701020 Copy

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7.	Rujitanaroj P O, Pimpha N, Supaphol P. Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles. Polymer (Guildf), 2008, 49(21): 4723-4732.
8.	Hong K H. Preparation and properties of electrospun poly(vinyl alcohol)/silver fiber web as wound dressings. Polym Eng Sci, 2007, 47(1): 43-49.
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11.	Buffet-Bataillon S, Tattevin P, Bonnaure-Mallet M, et al. Emergence of resistance to antibacterial agents: the role of quaternary ammonium compounds—a critical review. Int J Antimicrob Agents, 2012, 39(5): 381-389.
12.	Asri L A T W, Crismaru M, Roest S, et al. A shape-adaptive, antibacterial-coating of immobilized quaternary-ammonium compounds tethered on hyperbranched polyurea and its mechanism of action. Adv Funct Mater, 2014, 24(3): 346-355.
13.	Song J, Kong H, Jang J. Bacterial adhesion inhibition of the quaternary ammonium functionalized silica nanoparticles. Colloids Surf B Biointerfaces, 2011, 82(2): 651-656.
14.	Andresen M, Stenstad P, M?retr? T, et al. Nonleaching antimicrobial films prepared from surface-modified microfibrillated cellulose. Biomacromolecules, 2007, 8(7): 2149-2155.
15.	Woodruff M A, Hutmacher D W. The return of a forgotten polymer-polycaprolactone in the 21st century. Prog Polym Sci, 2010, 35(10): 1217-1256.
16.	Yang Fang, Both S K, Yang Xuechao, et al. Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application. Acta Biomater, 2009, 5(9): 3295-3304.
17.	Lee J, Tae G, Kim Y H, et al. The effect of gelatin incorporation into electrospun poly(L-lactide-co-epsilon-caprolactone) fibers on mechanical properties and cytocompatibility. Biomaterials, 2008, 29(12): 1872-1879.

1. Metcalfe A D, Ferguson M W. Bioengineering skin using mechanisms of regeneration and repair. Biomaterials, 2007, 28(34): 5100-5113.
2. Abrigo M, Mcarthur S L, Kingshott P. Electrospun nanofibers as dressings for chronic wound care: advances, challenges, and future prospects. Macromol Biosci, 2014, 14(6): 772-792.
3. Rieger K A, Birch N P, Schiffman J D. Designing electrospun nanofiber mats to promote wound healing—a review. J Mater Chem B, 2013, 1(36): 4531-4541.
4. Xue Jiajia, He Min, Liu Hao, et al. Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes. Biomaterials, 2014, 35(34): 9395-9405.
5. Torres-Giner S, Martinez-Abad A, Gimeno-Alca?iz J V, et al. Controlled delivery of gentamicin antibiotic from bioactive electrospun polylactide-based ultrathin fibers. Adv Eng Mater, 2012, 14(4): B112-B122.
6. Toncheva A, Paneva D, Maximova V, et al. Antibacterial fluoroquinolone antibiotic-containing fibrous materials from poly(L-lactide-co-D,L-lactide) prepared by electrospinning. Eur J Pharm Sci, 2012, 47(4): 642-651.
7. Rujitanaroj P O, Pimpha N, Supaphol P. Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles. Polymer (Guildf), 2008, 49(21): 4723-4732.
8. Hong K H. Preparation and properties of electrospun poly(vinyl alcohol)/silver fiber web as wound dressings. Polym Eng Sci, 2007, 47(1): 43-49.
9. Dashdorj U, Reyes M K, Unnithan A R, et al. Fabrication and characterization of electrospun zein/Ag nanocomposite mats for wound dressing applications. Int J Biol Macromol, 2015, 80: 1-7.
10. Lundin J G, Coneski P N, Fulmer P A, et al. Relationship between surface concentration of amphiphilic quaternary ammonium biocides in electrospun polymer fibers and biocidal activity. React Funct Polym, 2014, 77: 39-46.
11. Buffet-Bataillon S, Tattevin P, Bonnaure-Mallet M, et al. Emergence of resistance to antibacterial agents: the role of quaternary ammonium compounds—a critical review. Int J Antimicrob Agents, 2012, 39(5): 381-389.
12. Asri L A T W, Crismaru M, Roest S, et al. A shape-adaptive, antibacterial-coating of immobilized quaternary-ammonium compounds tethered on hyperbranched polyurea and its mechanism of action. Adv Funct Mater, 2014, 24(3): 346-355.
13. Song J, Kong H, Jang J. Bacterial adhesion inhibition of the quaternary ammonium functionalized silica nanoparticles. Colloids Surf B Biointerfaces, 2011, 82(2): 651-656.
14. Andresen M, Stenstad P, M?retr? T, et al. Nonleaching antimicrobial films prepared from surface-modified microfibrillated cellulose. Biomacromolecules, 2007, 8(7): 2149-2155.
15. Woodruff M A, Hutmacher D W. The return of a forgotten polymer-polycaprolactone in the 21st century. Prog Polym Sci, 2010, 35(10): 1217-1256.
16. Yang Fang, Both S K, Yang Xuechao, et al. Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application. Acta Biomater, 2009, 5(9): 3295-3304.
17. Lee J, Tae G, Kim Y H, et al. The effect of gelatin incorporation into electrospun poly(L-lactide-co-epsilon-caprolactone) fibers on mechanical properties and cytocompatibility. Biomaterials, 2008, 29(12): 1872-1879.

Journal of Biomedical Engineering

Non-leaching, broad-spectrum antibacterial poly (ε-caprolactone)/gelatin-QAS nanofiberous membranes for wound dressing

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